Nome
del laboratorio:
Unità di Endocrinologia, Dipartimento di Fisiopatologia Clinica,
Università degli Studi di Firenze, Viale Pieraccini, 6, 50139
Firenze
Responsabile:
Prof. Alessandro Peri, a.peri@dfc.unifi.it
Componenti:
Susanna Benvenuti, s.benvenuti@dfc.unifi.it;
Ilaria Cellai, ilaraia.cellai@virgilio.it;
Giovanna Danza, g.danza@dfc.unifi.it
Cristiana Deledda, delfam@libero.it;
Paola Luciani, p.luciani@dfc.unifi.it;
Fabiana Rosati, f.rosati@dfc.unifi.it
Linee di Ricerca:
- Neuroprotezione mediata dal gene seladin-1 in
modelli cellulari neuronali ;
- Lipid rafts e patologie neurodegenerative;
- GnRH , neuroprotezione e steroidogenesi
- Exendin e neuroprotezione;
- Basi molecolari della resistenza al trattamento
farmacologico nei tumori ipofisari
Modelli sperimentali utilizzati:
- Cellule mesenchimali staminali umane ottenute da
midollo osseo e da tessuto adiposo sottocutaneo;
- Colture a lungo termine di neuroblasti fetali
umani normali;
- Linee cellulari continue di neuroblastoma umano.
- Colture primarie di adenomi ipofisari umani
Metodologie utilizzate:
- Allestimento e mantenimento di colture cellulari
primarie e a lungo termine;
- Ottimizzazione di protocolli per il
differenziamento osteoblastico, adipocitario e neuronale di cellule
staminali mesenchimali;
- Saggi di proliferazione (MTS, 3H-thymidine uptake,
conta cellulare);
- Saggi di adesione cellulare;
- Immunocitochimica;
- Saggi di transfezione cellulare
- Real time PCR;
- Western blotting;
- PCR e RT-PCR;
- Analisi di sequenze genomiche per la ricerca di
sequenze responsive;
- Saggi di transattivazione trascrizionale;
- Ingegnerizzazione di plasmidi;
- Tecniche di silenziamento genico
Pubblicazioni (2004-2008):
- Luciani P., Ferruzzi P., Arnaldi G., Crescioli C.,
Benvenuti S., Nesi
G., Valeri A., Greeve I., Serio M., Mannelli M., Peri A. Expression of
the novel ACTH-responsive gene seladin-1/DHCR24 in the normal adrenal
cortex and in adrenocortical adenomas and carcinomas. J Clin Endocrinol
Metab, 2004, 89(3): 1332-1339.
- Cioppi, F., Simi, L., Luciani, P., Petraglia, F., Susini,
T., Cobellis,
L., Serio, M., Maggi, M., Peri, A. Expression of uteroglobin and matrix
metalloproteinase-9 genes in endometrial cancer: relationship to
estrogen and progesterone receptor status. Oncol Reports, 11:427-433,
2004
- Baglioni S, Corona G, Maggi M, Serio M, Peri A
Identification of a
novel mutation in the AVP-NPII gene affecting the sixth intrachain
disulfide bond of the neurophysin II moiety. Eur. J. Endocrinol.,
151:605-11, 2004
- Filopanti M, Ballarè E, Lania AG, Bondioni S, Verga
U, Locatelli
M, Zavanone LM, Losa M, Gelmini S, Peri A, Orlando C, Beck-Peccoz P,
Spada A. Loss of heterozygosity at the SS receptor type 5 locus in
human GH- and TSH-secreting pituitary adenomas. J Endocrinol Invest,
27:937-942, 2004
- E.G. Occhiato, A. Ferrali, G. Menchi, A. Guarna, G. Danza,
A. Comerci,
R. Mancina, M. Serio, G. Garotta, A. Cavalli, M De Vivo, M. Recanatini.
“Synthesis, biological activity, and three-dimensional quantitative
structure-activity relationship model for a series of
benzo[c]quinolizin-3-ones, nonsteroidal inhibitors of human steroid
5alpha-reductase 1.” J Med Chem. 47(14), 2004, 3546-60.
- E.G. Occhiato, A. Guarna, G. Danza, M. Serio: “Selective
nonsteroidal
inhibitors of 5a-Reductase Type 1” J. Steroid Biochem. Mol. Biol.
88(1), 2004, 1-16.
- Peri A, Danza G, Serio M. Seladin-1 as a target of estrogen
receptor
activation in the brain: a new gene for a rather old story? J
Endocrinol Invest, 28:285-293, 2005.
- Ferrali, G. Menchi, E.G. Occhiato, G. Danza, R. Mancina, M.
Serio, A.
Guarna : “Synthesis and activity of 8-substituted
benzo[c]quinolizin-3-ones as dual inhibitors of human 5alpha-reductases
1 and 2.” Bioorg Med Chem Lett. 15(1), 2005, 145-8
- F. Rosati, I. Bardazzi, P. De Blasi, L. Simi, D. Scarpi, A.
Guarna, M.
Serio, M.L. Racchi, G. Danza. “5alpha-Reductase activity in
Lycopersicon esculentum: cloning and functional characterization of
LeDET2 and evidence of the presence of two isoenzymes.” J Steroid
Biochem Mol Biol. 96(3-4), 2005, 287-99.
- Filopanti M, Ronchi C, Ballare E, Bondioni S, Lania AG,
Losa M, Gelmini
S, Peri A, Orlando C, Beck-Peccoz P, Spada A.Analysis of somatostatin
receptor 2 and 5 polymorphisms in patients with acromegaly. J Clin
Endocrinol Metab., 90:4824-8, 2005.
- Benvenuti S., Luciani P., Vannelli G.B., Gelmini S.,
Franceschi E.,
Serio M., Peri A.: Estrogen and selective estrogen receptor modulators
exert neuroprotective effects and stimulate the expression of selective
Alzheimer’s disease indicator-1, a recently discovered antiapoptotic
gene, in human neuroblast long-term cell cultures. J Clin Endocrinol
Metab, 2005, 90(3): 1775-1782.
- Luciani P, Gelmini S, Ferrante E, Lania A, Benvenuti S,
Baglioni S,
Mantovani G, Cellai I, Ammannati F, Spada A, Serio M, Peri A.
Expression of the antiapoptotic gene seladin-1 and octreotide-induced
apoptosis in growth hormone-secreting and nonfunctioning pituitary
adenomas. J Clin Endocrinol Metab. 2005 Nov;90(11):6156-61
- Valeri A, Bergamini C, Manca G, Mannelli M, Presenti L,
Peri A,
Borrelli A, Tonelli L. Adrenal incidentaloma: the influence of a
decision-making algorithm on the short-term outcome of laparoscopy. J
Laparoendoscopic & Adv Surg Tech., 15:451-9, 2005.
- Parenti G, Nassi R, Silvestri S, Bianchi S, Valeri A, Manca
G,
Mangiafico S, Ammannati F, Serio M, Mannelli M, Peri A. Multi-step
approach in a complex case of Cushing’s syndrome and medullary thyroid
carcinoma J Endocrinol Invest, 29:177-81, 2006
- Biliotti GC, Martini F, Vezzosi V, Seghi P, Tozzi F,
Castagnoli A,
Basili G, Peri A Specific features of differentiated thyroid carcinoma
in patients over 70 years of age. J Surg Oncol, 93:194-198, 2006
- Benvenuti S, Saccardi R, Luciani P, Urbani S, Deledda C,
Cellai I,
Francini F, Squecco R, Rosati F, Danza G, Gelmini S, Greeve I, Rossi M,
Maggi R, Serio M, Peri A.: Neuronal differentiation of human
mesenchymal stem cells: changes in the expression of the Alzheimer's
disease-related gene seladin-1. Exp Cell Res. 2006 Aug
1;312(13):2592-604.
- Cellai I, Benvenuti S, Luciani P, Galli A, Ceni E, Simi L,
Baglioni S,
Muratori M, Ottanelli B, Serio M, Thiele CJ, Peri A. : Antineoplastic
effects of rosiglitazone and PPARgamma transactivation in neuroblastoma
cells. Br J Cancer. 2006 Oct ;95(7):879-88. Epub 2006 Sep 12.
- Zogheri A, Di Mambro A, Mannelli M, Serio M, Forti G, Peri
A
Hyponatremia and pituitary adenoma: think twice about the
etiopathogenesis. J Endocrinol Invest, 29:750-53, 2006
- Ferrante E, Pellegrini C, Gamba B, Rampini P, Locatelli
M,
Filopanti M, Corbetta S, Peri A, Gelmini S, Bosari S, Mantovani G,
Beck-Peccoz P, Spada A. Lania A. Octreotide promotes apoptosis in human
GH secreting adenomas by activating somatostatin receptor type 2.
Endocrine Related Cancer, 13:955-62, 2006
- Parenti G, Ricca V, Zogheri A, Mannelli M, Serio M, Peri A
A case of
hyponatremia due to isolated ACTH deficit. Nature Cl Pr Endocrinol
Metab, 3:369-75, 2007
- Benvenuti S, Cellai I, Luciani P, Deledda C, Baglioni S,
Giuliani C,
Saccardi R, Mazzanti B, Dal Pozzo S, Mannucci E, Peri A, Serio M. :
Rosiglitazone stimulates adipogenesis and decreases osteoblastogenesis
in human mesenchymal stem cells. J Endocrinol Invest. 2007
Oct;30(9):RC26-30.
- Kuehnle K, Crameri A, Kälin RE, Luciani P, Benvenuti
S, Peri A,
Ratti F, Rodolfo M, Kulic L, Heppner FL, Nitsch RM, Mohajeri MH.:
Pro-survival Effect of DHCR24/seladin-1 in an Acute and a Chronic
Response to Oxidative Stress. Mol Cell Biol. 2007 Nov 5.
- Tedde A, Cellini E, Bagnoli S, Sorbi S, Peri A, Mutational
screening
analysis of DHCR24/seladin-1 gene in Italian familial Alzheimer’s
disease. Am J Med Genetics PartB, 147:117-119, 2008.
- Filopanti M, Barbieri AM, Angioni AR, Colao A, Gasco V,
Grottoli S,
Peri A, Baglioni S, Faustini Fustini M, Pigliaru F, Del Monte P,
Borretta G, Ambrosi B, Jaffrain-Rea ML, Gasperi M, Brogioni S ,
Mantovani G, Beck-Peccoz P, Lania A, Spada A. Dopamine D2 receptor gene
polymorphisms and response to Cabergoline therapy in patients with
prolactin-secreting pituitary adenomas. Pharmacogenomics J, 2008 Mar
11; [Epub ahead of print]. (IF 3.957)
- Cecchi C, Rosati F, Pensalfini A, Formigli L, Nosi D,
Liguri G,
Dichiara F, Morello M, Danza G, Pieraccini G, Peri A, Serio M,
Stefani M , Seladin-1/DHCR24 protects neuroblastoma cells against
amyloid toxicity by increasing membrane cholesterol content.J Cell Mol
Med, 2008 Jan 11; [Epub ahead of print]
- Benvenuti S.*, Luciani P.*, Cellai I., Deledda, C.,
Baglioni S.,
Saccardi R, Urbani S., Francini F., Squecco R., Giuliani C.,
Vannelli G.B., Serio M., Pinchera A., Peri A. “Thyroid hormones
promote cell differentiation and up-regulate the expression of
seladin-1 gene in in vitro models of human neuronal precursors”.
Journal of Endocrinology. In press
- A. Peri, I. Cellai, S. Benvenuti, P. Luciani, S. Baglioni,
M.
Serio. "PPARgamma in neuroblastoma" Review Article PPAR research.
Accepted, revised
- P. Luciani, C. Deledda, F. Rosati, S. Benvenuti, I. Cellai,
F.
Dichiara, M. Morello, G. B. Vannelli, G. Danza, M. Serio, A. Peri.
Seladin-1 is a fundamental mediator of the neuroprotective effects of
estrogen in human neuroblast long-term cell cultures, Endocrinology #
EN-07-1795 Version 1, under revision
- S. Giannini, A. Pezzatini, P. Luciani, C. Manuelli, S.
Benvenuti, I.
Cellai, C. Deledda, G.B. Vannelli, C.M. Rotella, M. Serio, A. Peri.
“Intermittent high glucose concentrations reduce neuronal precursor
proliferation by alterino the IGF system: the involvement of the
neuroprotective factor seladin-1”. Journal of Endocrinology, under
revision
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