Nome
del laboratorio:
Laboratorio dell’U.O. di Endocrinologia e Diabetologia
Dipartimento di Scienze Mediche
Università di Milano
Fondazione Ospedale Maggiore IRCCS - Milano
Responsabile:
Prof.ssa Anna Spada
anna.spada@unimi.it
Componenti:
Dott. Andrea Lania
andrea.lania@unimi.it
Dott.ssa Giovanna Mantovani
giovanna.mantovani@unimi.it
Dott.ssa Sara Bondioni
sara.bondioni@unimi.it
Dott.ssa Erika Peverelli
erika.peverelli@unimi.it
Dott.ssa Annamaria Barbieri anna.barbieri@unimi.it
Antonia Maffini
Luca Olgiati
Linee di Ricerca:
- eziopatogenesi tumori ipofisari
- studio del signalling coinvolto nel controllo dei
processi proliferativi ed antiproliferativi nei tumori ipofisari
- studio dei processi di internalizzazione e
desensitizzazione dei recettori della somatostatina e della dopamina
- studio del ruolo delle varianti polimorfiche dei
GPCR nella patogenesi dei tumori ipofisari e nella risposta ai
trattamenti farmacologici
Modelli sperimentali utilizzati
e metodologie utilizzate:
- Estrazione proteine e acidi nucleici da tessuto o
colture cellulari
- Colture cellulari (colture primarie da tumori
ipofisari umani chirurgicamente rimossi e linee cellulari: GH3, HP75,
CHO)
- Western blot
- PCR e sequenziamento genico
- Analisi di metilazione
- Produzione di costrutti per trasfezioni
- Trasfezioni stabili e transienti
- Silenziamento genico (siRNA)
- Analisi di proliferazione e apoptosi di cellule in
coltura
- Analisi in microscopia in fluorescenza e confocale
- Dosaggi ormonali con metodiche radioattive e non
Pubblicazioni selezionate
(2004-2008):
- Bondioni S, Angioni AR, Corbetta S, Locatelli M, Ferrero S,
Ferrante E,
Mantovani G, Olgiati L, Beck-Peccoz P, Spada A, Lania AG. Effect of
9-cis Retinoic Acid on Dopamine D2 Receptor Expression in Pituitary
Adenoma Cells. Exp Biol Med (Maywood). 2008 Apr;233(4):439-46.
- Filopanti M, Barbieri AM, Angioni AR, Colao A, Gasco V,
Grottoli S,
Peri A, Baglioni S, Fustini MF, Pigliaru F, Monte PD, Borretta G,
Ambrosi B, Jaffrain-Rea ML, Gasperi M, Brogioni S, Cannavò S,
Mantovani G, Beck-Peccoz P, Lania A, Spada A. Dopamine D(2)
receptor gene polymorphisms and response to cabergoline therapy
in patients with prolactin-secreting pituitary adenomas.
Pharmacogenomics J. 2008 Mar 11; [Epub ahead of print]
- Peverelli E, Mantovani G, Calebiro D, Doni A, Bondioni S,
Lania A,
Beck-Peccoz P, Spada A. The third intracellular loop of the human
somatostatin receptor 5 is crucial for arrestin binding and
receptor internalization after somatostatin stimulation. Mol
Endocrinol. 2008 Mar;22(3):676-88.
- Bondioni S, Mantovani G, Polentarutti N, Ambrosi B, Loli P,
Peverelli
E, Lania AG, Beck-Peccoz P, Spada A.Evaluation of proopiomelanocortin
mRNA in the peripheral blood from patients with Cushing's syndrome of
different origin. J Endocrinol Invest. 2007 Nov;30(10):828-32.
- Mantovani G, Bondioni S, Lania AG, Rodolfo M, Peverelli E,
Polentarutti
N, Veliz Rodriguez T, Ferrero S, Bosari S, Beck-Peccoz P, Spada A. High
expression of PKA regulatory subunit 1A protein is related to
proliferation of human melanoma cells. Oncogene. 2007 Oct 1;
[Epub ahead of print]
- Mantovani G, Lania AG, Bondioni S, Peverelli E, Pedroni C,
Ferrero S,
Pellegrini C, Vicentini L, Arnaldi G, Bosari S, Beck-Peccoz P, Spada
A.Different expression of protein kinase A (PKA) regulatory subunits in
cortisol-secreting adrenocortical tumors: relationship with cell
proliferation. Exp Cell Res. 2008 Jan 1;314(1):123-30.
- Giavoli C, Bergamaschi S, Ferrante E, Ronchi CL, Lania AG,
Rusconi R,
Spada A, Beck-Peccoz P. Effect of growth hormone deficiency and
recombinant hGH (rhGH) replacement on the
hypothalamic-pituitary-adrenal axis in children with idiopathic
isolated GH deficiency. Clin Endocrinol (Oxf). 2008 Feb;68(2):247-51.
- Mantovani G, Bondioni S, Linglart A, Maghnie M, Cisternino
M, Corbetta
S, Lania AG, Beck-Peccoz P, Spada A. Genetic analysis and evaluation of
resistance to thyrotropin and growth hormone-releasing hormone in
pseudohypoparathyroidism type Ib. J Clin Endocrinol Metab. 2007
Sep;92(9):3738-42. Epub 2007 Jun 26.
- Barbieri AM, Filopanti M, Bua G, Beck-Peccoz P.Two novel
nonsense
mutations in GALNT3 gene are responsible for familial tumoral
calcinosis. J Hum Genet. 2007;52(5):464-8. Epub 2007 Mar 10.
- Ferrante E, Pellegrini C, Bondioni S, Peverelli E,
Locatelli M, Gelmini
P, Luciani P, Peri A, Mantovani G, Bosari S, Beck-Peccoz P, Spada A,
Lania A.Octreotide promotes apoptosis in human somatotroph tumor cells
by activating somatostatin receptor type 2. Endocr Relat Cancer. 2006
Sep;13(3):955-62.
- Mantovani G, De Menis E, Borretta G, Radetti G, Bondioni S,
Spada A,
Persani L, Beck-Peccoz P. DAX1 and X-linked adrenal hypoplasia
congenita: clinical and molecular analysis in five patients. Eur J
Endocrinol. 2006 May;154(5):685-9.
- Angioni AR, Lania A, Cattaneo A, Beck-Peccoz P, Spada A.
Effects of
chronic retinoid administration on pituitary function. J Endocrinol
Invest. 2005 Dec;28(11):961-4.
- Mantovani G, Asteria C, Pellegrini C, Bosari S, Alberti L,
Bondioni S,
Peverelli E, Spada A, Beck-Peccoz P. HESX1 expression in human normal
pituitaries and pituitary adenomas. Mol Cell Endocrinol. 2006 Mar
9;247(1-2):135-9. Epub 2006 Jan 18. Erratum in: Mol Cell Endocrinol.
2006 Jun 22;251(1-2):112.
- Mantovani G, Mancini M, Gazzano G, Spada A, Colpi GM,
Beck-Peccoz P,
Persani L. Somatic mutational analysis of DAX1 in testes from men
with idiopathic azoospermia. Fertil Steril. 2005 Nov;84(5):1542-4.
- Mantovani G, Bondioni S, Ferrero S, Gamba B, Ferrante E,
Peverelli E,
Corbetta S, Locatelli M, Rampini P, Beck-Peccoz P, Spada A, Lania AG.
Effect of cyclic adenosine 3',5'-monophosphate/protein kinase a pathway
on markers of cell proliferation in nonfunctioning pituitary adenomas.
J Clin Endocrinol Metab. 2005 Dec;90(12):6721-4. Epub 2005 Oct 4.
- Calebiro D, de Filippis T, Lucchi S, Covino C, Panigone S,
Beck-Peccoz
P, Dunlap D, Persani L. Intracellular entrapment of wild-type TSH
receptor by oligomerization with mutants linked to dominant TSH
resistance. Hum Mol Genet. 2005 Oct 15;14(20):2991-3002. Epub 2005 Aug
31.
- Peverelli E, Mantovani G, Bondioni S, Pellegrini C, Bosari
S, Lania AG,
Beck-Peccoz P, Spada A. Expression of the two alternatively spliced
PRKAR1A RNAs in human endocrine glands. Mol Cell Endocrinol. 2005 Jun
30;238(1-2):51-5.
- Filopanti M, Ronchi C, Ballarè E, Bondioni S, Lania
AG, Losa M,
Gelmini S, Peri A, Orlando C, Beck-Peccoz P, Spada A. Analysis of
somatostatin receptors 2 and 5 polymorphisms in patients with
acromegaly. J Clin Endocrinol Metab. 2005 Aug;90(8):4824-8. Epub 2005
May 24.
- Filopanti M, Ballarè E, Lania AG, Bondioni S, Verga
U, Locatelli
M, Zavanone LM, Losa M, Gelmini S, Peri A, Orlando C, Beck-Peccoz
P, Spada A. Loss of heterozygosity at the SS receptor type 5 locus in
human GH- and TSH-secreting pituitary adenomas. J Endocrinol Invest.
2004 Nov;27(10):937-42.
- Lania AG, Mantovani G, Ferrero S, Pellegrini C, Bondioni S,
Peverelli
E, Braidotti P, Locatelli M, Zavanone ML, Ferrante E, Bosari S,
Beck-Peccoz P, Spada A. Proliferation of transformed somatotroph cells
related to low or absent expression of protein kinase a regulatory
subunit 1A protein. Cancer Res. 2004 Dec 15;64(24):9193-8.
- Mantovani G, Bondioni S, Locatelli M, Pedroni C, Lania AG,
Ferrante E,
Filopanti M, Beck-Peccoz P, Spada A. Biallelic expression of the
Gsalpha gene in human bone and adipose tissue. J Clin Endocrinol Metab.
2004 Dec;89(12):6316-9.
- Arosio M, Ronchi CL, Beck-Peccoz P, Gebbia C, Giavoli C,
Cappiello V,
Conte D, Peracchi M. Effects of modified sham feeding on ghrelin levels
in healthy human subjects. J Clin Endocrinol Metab. 2004
Oct;89(10):5101-4.
- Lania A, Mantovani G, Ferrante E, Zavanone LM, Locatelli M,
Corbetta S,
Beck-Peccoz P, Spada A. Gonadotropin-releasing hormone initiates
multiple signaling pathways in human GH-secreting adenomas. J
Endocrinol Invest. 2004 Apr;27(4):328-33.
- Mantovani G, Bondioni S, Lania AG, Corbetta S, de Sanctis
L, Cappa M,
Di Battista E, Chanson P, Beck-Peccoz P, Spada A. Parental origin of
Gsalpha mutations in the McCune Albright syndrome and in isolated
endocrine tumors. J Clin Endocrinol Metab. 2004 Jun;89(6):3007-9.
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